Seahorse XF analyzer

The Agilent Seahorse XF analyzer, a series of advanced metabolic assay platforms offers cell analysis technology solutions, facilitating researchers in detecting discrete alterations in cellular bioenergetics in real time. With improved precision, and accurate measurements Offering valuable insights into the pivotal functions driving cell signaling, proliferation, activation, toxicity, and biosynthesis.

Additionally, the platform incorporates pharma-oriented workflows, specifically designed to cater to the needs of drug development and discovery.

Key advantages: 

• Enables robust measurements of mitochondrial activity, glycolysis, and ATP production rates in a microplate format.
• Provides automatic calculation of oxygen consumption and proton efflux rates.
• Allows for label-free detection of discrete bioenergetic changes in live cells—in real time.
• Compatible with both adherent and suspension cells, as well as isolated mitochondria.
• Instrument-controlled gentle mixing, measuring, and compound injections for utmost accuracy.
• High sensitivity that allows the analysis of as few as 5,000 cells per well.
• Temperature-controlled to maintain cell health and kinetics.

Applications

Cellular Metabolism

The Seahorse XF platform is a key tool in analyzing cellular metabolism by measuring the two main metabolic pathways, mitochondrial respiration, and glycolysis, in live cells in real-time. 

Immune Metabolism

Metabolic reprogramming drives critical immune cell processes such as activation, proliferation, and memory development. The Seahorse XF platform uncovers these intricate metabolic processes in real time.

Cancer Metabolism

The Seahorse XF Analyzer aids in revealing cancer metabolic liabilities through functional bioenergetic analysis of live cells in real time.